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Knockdown of target genes

SG Sharath Chandra Gaddelapati
SG Smitha George
AM Anilkumar Moola
KS Karthi Sengodan
SP Subba Reddy Palli
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Gene fragments ranging from 300 to 500 bp were amplified using gene-specific primers (Supplementary Table 5). These primers were designed to incorporate T7 promoter sequences at the 5’ end. cDNA was used as a template for amplifying target genes. Double-stranded RNA (dsRNA) was synthesized using the MEGAscript T7 kit (Catalog No: A57622, Invitrogen, USA) and the PCR product was purified following the manufacturer’s instructions. Newly molted last instar larvae were microinjected with 1 μg of cognate dsRNA. While control larvae were injected with dsmalE: dsRNA targets the gene coding for a maltose-binding protein of E. coli.

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