Improve Research Reproducibility A Bio-protocol resource
Concise Method
tooltip

DNA extraction and genotyping by sequencing analysis

RB Rind Balech
FM Fouad Maalouf
SK Sukhjiwan Kaur
AJ Abdulqader Jighly
RJ Reem Joukhadar
AA Alsamman M. Alsamman
AH Aladdin Hamwieh
LK Lynn Abou Khater
DR Diego Rubiales
SK Shiv Kumar
request Request a Protocol
ask Ask a question
Favorite

DNA was extracted from young leaves of seedlings aged between 4 and 6 weeks, prior to the application of salt treatment, using the CTAB method, as outlined by Rogers and Bendich77. A total of 50 μl of 100 ng/μl DNA from each sample was sent to Agriculture Victoria, Melbourne, where Multispecies Pulse SNP chip was used for genotyping. To ensure the quality of the markers, we filtered them by call rates greater than 80%, minor allele frequency (MAF) of ≥ 5%, and heterozygosity of ≤ 15%. Only those markers that met these criteria were selected for genome-wide association analysis.

Copyright and License information: The Author(s) ©2024
Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

post Post a Question
0 Q&A