In vivo BrdU incorporation assay

SR Sayoni Roy
DM Darshan Mehta
AP Akshay Paradkar
GC Gopal Chovatiya
SW Sanjeev K. Waghmare
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BrdU was injected intraperitoneally with BrdU (50 mg/kg of body weight) at PD30 accompanied by 0.8 mg/ml BrdU in water for three days (PD30–32). Mice were sacrificed and skin sections were collected at PD32. BrdU was exposed by treating the tissue sections with 2 N HCL for 1 h at 37 °C and detected by the anti-BrdU antibody through IFA. (Supplementary Data 4).

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