Mouse liver was homogenized in extemporaneously prepared isolation buffer (10 mM Tris/MOPS; 1 mM EGTA/Tris; 200 mM sucrose; pH 7.4) using a Dounce homogenizer. After centrifugation at 600 × g for 10 min at 4 °C, the collected supernatant was centrifuged at 7000 × g for 10 min at 4 °C. The pellet containing mitochondria was resuspended in 1 mL isolation buffer. Mitochondria were quantified using the DC protein assay (Bio-Rad, Marnes-la-Coquette, France) and resuspended at 10 μg/50 μL in extemporaneously prepared mitochondrial assay solution: 70 mM sucrose; 220 mM mannitol; 10 mM KH2PO4; 5 mM MgCl2; 2 mM HEPES; 1 mM EGTA; BSA Fatty acid free 0.2%; pH 7.4).
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