2.4. HSMM, C2C12 cell culture

Human skeletal muscle myoblasts (HSMMs) were obtained from Lonza. HSMM were cultured at 37 °C in a humidified atmosphere with 5% CO₂ in SkGM-2 medium (Lonza, MD, USA).

Mouse muscle myoblasts, C2C12 (ATCC, VA, USA) were cultured in DMEM medium supplemented with 10% FBS. For differentiation, when fully confluent, the medium was changed to a differentiation medium consisting of DMEM supplemented with 2% HS.