Improve Research Reproducibility A Bio-protocol resource
- Home
- Protocols
-
Request a Protocol
Ask a
question
Favorite
Hep3B cells were seeded in six-well plates and cultured under standard growth conditions for 24 h. After the cells reached 60–70% confluence, the culture medium was removed, and the cells were mock infected or infected with DENV 2 for 2 h, after which the virus inoculum was removed and various concentration of metformin (Merck Group, Darmstadt, Germany) were added into the cells in normal growth medium and cells were then further incubated under standard conditions for 24 h. All experiments were undertaken as three independent biological three replicates.
Copyright and License information: The Author(s) ©2024
Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.
Post a Question0 Q&A
