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The genomic DNA were amplified using extracted genomic DNA as template. The PCR products were annealed in 10× Reaction buffer by heating to 95 °C for 5 min, followed by a 2 °C/s ramp down to 85 °C and a 0.1 °C/s ramp down to 25 °C. The annealed samples were digested by T7 Endonuclease I (Beyotime) at 37 °C for 30 min, followed by incubating at 85 °C for 15 min to stop the reaction.
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