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The protein level detection of cytokines
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Plasma cytokine concentrations of IL-17A, IL-6, IL-22, and CCL-20 were determined by a commercial ELISA kit (Dogesce). For skin IL-17A detection, the back skin of mice was isolated and triturated with a grinder (Tiss-24, Jing Xin). Cell debris was precipitated via centrifugation (1000×g, 4 °C, 15 min), and IL-17A was measured in the supernatant using the IL-17A ELISA kit (Dogesce) and normalized to the total protein concentration, which was determined via BCA assay.
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