Toxicity evaluation of PD-L1-Fc-loaded nanoparticles

On the 10th day of DSS treatment, healthy female mice and DSS-induced mice subjected to saline, Blank/Oxi-αCD nanoparticles, PD-L1-Fc protein or PD-L1-Fc/Oxi-αCD nanoparticles were euthanized. The major organs, including the heart, liver, spleen, lungs, kidneys, stomach and intestine, were isolated and stained with H&E.

In another cohort study, mice were randomly divided into two groups. The experimental group was intraperitoneally injected with PD-L1-Fc/Oxi-αCD nanoparticles at a dose of 5.0 g/kg. The control group was intraperitoneally injected with saline. The experimental mice and control mice were bred separately in different cages. After two weeks, the mice were euthanized. The major organs, including the colon, heart, liver, spleen, lung, kidney, stomach, and intestine, were isolated. Histopathological sections were prepared and stained with H&E.