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4.9. Real-Time PCR

MJ Michihisa Jougasaki
YT Yoko Takenoshita
KU Katsuyuki Umebashi
MY Masayoshi Yamamoto
KS Ku Sudou
HN Hitoshi Nakashima
MS Masahiro Sonoda
TK Tamahiro Kinjo
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Semi-quantitative real-time-PCR was performed using Power SYBR Green PCR Master Mix (Applied Biosystems, Warrington, UK) on a CFX connect thermal cycler (Bio-Rad, Hercules, CA, USA). The value of each cDNA was calculated using the ΔΔCq method and normalized to the value of GAPDH.

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Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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