Primary plating and isolation

DC Debjit Chakraborty
FD Falguni Debnath
AM Agniva Majumdar
AC Atreyi Chakrabarti
MS Monica Sharma
KW Kamini Walia
AD Alok Kumar Deb
SD Shanta Dutta
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The urine sample was thoroughly mixed and then plated on CLED agar using the standard loop method (nichrome loop having diameter 4 mm–0.01 ml) and placed in a bacteriological incubator set at 37 °C for 18 to 24 h incubation. Colony count was calculated to determine the presence of significant bacteriuria21,22.

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