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Isolation and ecology

CB Carrie Brady
SK Sundeep Kaur
BC Bridget Crampton
DM Daniel Maddock
DA Dawn Arnold
SD Sandra Denman
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Bacterial strains were isolated from the bleeding cankers of Platanus x acerifolia (London plane) at St James Park, London and Tilia x europaea (common lime) at Tidworth Garrison, Wiltshire. Swab samples of the weeping exudate were collected from the London plane in May and October, 2020. A small bark panel (3 × 3 cm) was taken from the bleeding common lime in August 2019.

Swabs were rehydrated in sterile 1/4 Ringers, the swab spread on Luria-Bertani (LB) agar (Oxoid) and Eosin Methylene Blue (EMB) agar (Merck) which were initially incubated at 35°C under anaerobic conditions for 4 days to promote growth of facultatively anaerobic bacteria. Bark tissue was removed from the panel with a scalpel to expose the developing lesion. Wood chips were cut around the advancing lesion front, plated on LB agar and incubated at 25°C for 7 days. Single colonies were obtained through re-streaking on LB agar and incubation at 25°C.

The strains used in this study are listed in Supplementary Table 1.

Copyright and License information:  ©2022 Brady, Kaur, Crampton, Maddock, Arnold and Denman.
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