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2.4. Two Types of LEC Aging Model In Vitro
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For natural aging model, mouse LECs were passed weekly, and medium was changed twice a week. LECs at passages 4 and 15 were defined as young cells and senescent cells, respectively.
For H2O2-induced aging model, cells were incubated with 100 μM H2O2 for 1 week. Fresh H2O2-containing medium was changed every day. Senescent cells were characterized as positive SA-β-gal staining and prolonged population doubling times.
Copyright and License information: ©2022 Yang Huang et al.
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