Cell Transfection

A negative control (NC), miR-320a mimics, and miR-320a inhibitor were obtained from General Biol (Anhui, China) and transfected into Jurkat cells using Lipofectamine^® 2000 (Invitrogen, Carlsbad, CA, USA) referring to the manual. Then, a lncRNA Smart Silencer for human LINC00680 was purchased from Ribobio (Guangdong, China). Lipofectamine^® 2000 was selected to transfect Jurkat cells with specific plasmids, as described previously. The sense and antisense sequences for the LINC00680 Smart Silencers were as follows: siLINC00680-1, 5′-TCCATTCATTGGGAAATCA-3′ and 5′-AGGGCAGTGTGGAGTGACA-3′; siLINC00680-2, 5′-CATGGACAATATCATAGTT-3′ and 5′-CCTCAGCTCTCCATGGCTCT-3′; and siLINC00680-3, 5′-AGTGTGGAGTGACAGGCACG-3′ and 5′-AAGCATCCATTCATTGGGAA-3′. Cells were cultured in a humidified atmosphere of 5% CO₂ at 37°C. Total RNA was then extracted from the transfected cells and analyzed by qRT-PCR to assess the transfection efficiency.