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Construction of protein–protein interaction networks
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DEGs from musculoskeletal aging and AD samples were employed to construct two unique networks of encoded proteins using The Search Tool for the Retrieval of Interacting Genes (STRING)18. The protein–protein interactions (PPI) within the two networks were inferred using a medium probabilistic confidence score of > 0.4 and predicted with Cytoscape19. The use of a moderate cut-off was ensued to increase the coverage of all possible protein interactions without overestimating their precision. Non-interacting proteins were excluded from the networks.
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