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Characterization of EVs
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hBMSCs (hTERT-BMSC clone Y201), purchased from BNBIO (http://www.bnbio.com/pro/p3/16/p_35521.html), were cultured with high-glucose Dulbecco’s Modified Eagle’s medium (DMEM; Solarbio, Beijing, China) supplemented with 10% fetal bovine serum (FBS) in a 37 °C incubator with 5% CO2.
Flow cytometry showed that hBMSCs were positive for CD29 (ab21845), CD44 (ab25024), and CD73 (ab239246) while hematopoietic markers, such as CD34 (ab18224), CD45 (ab27287), and HLA-DR (ab1182) were absent. All antibodies were purchased from Abcam Inc. (Cambridge, MA).
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