Lifespan measurements

Lifespans were carried out as previously described with minor modifications⁷³. Briefly, 20–30 gravid adult animals were placed on NGM plates for a timed egg-lay. After 12–16 h, these animals were removed. Once their progeny reached late L4/early adult stage, animals were transferred to plates with 33 µL of 150 mM fluorodeoxyuridine (FUdR) and 100 µL of 50 mg/mL Ampicillin per 100 mL NGM to prevent the development of progeny and growth of bacteria. Roughly 75 worms were placed on each NGM + FUdR plate seeded with concentrated bacteria (×5). A minimum of two plates per strain per condition were used per replicate. Lifespan plates were transferred periodically during early adulthood to prevent starvation and avoid contamination. Animals were scored as dead and removed from the experiment when they did not move in response to prodding under a dissection microscope. It is notable that FUdR can extend lifespan and is a confounding factor contributing to lifespan extension⁷⁴, however, to avoid matricide under DR, we include FUdR in the lifespan plates in this study. This factor should be considered when interpreting the results.