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Comparison of protein sequences among ruminants

PM Pauline Martin
IP Isabelle Palhière
CM Cyrielle Maroteau
PB Philippe Bardou
KC Kamila Canale-Tabet
JS Julien Sarry
FW Florent Woloszyn
JB Justine Bertrand-Michel
IR Ines Racke
HB Hüseyin Besir
RR Rachel Rupp
GT Gwenola Tosser-Klopp
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The sequence of the goat DGAT1 gene was then translated and the corresponding protein sequence was compared to those of other ruminants, The sheep and bovine sequences were extracted from Uniprot (http://www.uniprot.org/ entries A8VJM4 and Q8MK44 respectively). The weblogo software (http://weblogo.threeplusone.com/) was used to obtain a graphical representation of the conservation of the protein between three domestic ruminant (cattle, sheep and goats). Predicted protein sequences of other ruminant species (Ceratotherium simum; Bulbalus bubalis; Camelus dromedaries; Camelus bactrianus and Vicugna_pacos) were extracted from NCBI (http://www.ncbi.nlm.nih.gov/gene/?term=dgat1). A percent identity matrix was then created between all the species retained using the Clustal omega software (http://www.ebi.ac.uk/Tools/msa/clustalo/).

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