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BRCA1 promoter hypermethylation

BP Benedetta Pellegrino
AH Andrea Herencia-Ropero
AL Alba Llop-Guevara
FP Flaminia Pedretti
AM Alejandro Moles-Fernández
CV Cristina Viaplana
GV Guillermo Villacampa
MG Marta Guzmán
OR Olga Rodríguez
JG Judit Grueso
JJ Jose Jiménez
EA Enrique J. Arenas
AD Andrea Degasperi
JD João M. L. Dias
JF Josep V. Forment
MO Mark J. O’Connor
OD Olivier Déas
SC Stefano Cairo
YZ Yinghui Zhou
AM Antonino Musolino
CC Carlos Caldas
SN Serena Nik-Zainal
RC Robert B. Clarke
PN Paolo Nuciforo
OD Orland Díez
XS Xavier Serres-Créixams
VP Vicente Peg
ME Martín Espinosa-Bravo
TM Teresa Macarulla
AO Ana Oaknin
JM Joaquin Mateo
JA Joaquín Arribas
RD Rodrigo Dienstmann
MB Meritxell Bellet
MO Mafalda Oliveira
CS Cristina Saura
SG Sara Gutiérrez-Enríquez
JB Judith Balmaña
VS Violeta Serra
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BRCA1 promoter methylation was measured using methylation-specific multiplex ligation-dependent probe amplification (MS-MLPA; MRC Holland, Amsterdam, the Netherlands) according to manufacturer’s instructions. Two of the xenografts generated in CRUK/UCAM (STG139 and STG201) had been previously tested using reduced-representation bisulfite sequencing (RRBS)(16) and further validated using MS-MLPA. Positive controls of BRCA1 promoter hypermethylation were used (T127 and/162).(24)

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