Chemicals

Dimethyl sulfoxide (DMSO, D8418), formaldehyde (61783750001730), and isopropanol (1.94524.2521) were purchased from Merck Ltd., Mumbai, India. 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT, M2128), neutral red uptake (NRU, N4638), sulpho rhodamine B (SRB, 230162), antibiotic-antimycotic (Ab/Am, A5955) solution, HEPES (H4034), Propidium iodide (PI, P4170), RNase A (R5000), DCF-DA (D6883), phosphate-buffered saline (PBS, P3813), Rhodamine 123 (R8004), trypsin (93615), ODC (O3001), CATD (C8696), hemoglobin (H2625), sodium bicarbonate (S6297), dihydrofolate reductase (D6566), HYAL (H3884), hyaluronic acid (HA, 924474), cyclooxygenase-2 (COX-2, C0858), methotrexate (MTX, 06563), pepstatin A (P4265), α-difluoro methyl ornithine (DFMO, D193), N-acetyl cysteine (NAC, A7250), celecoxib (32097), zileuton (Z4277), and carvacrol (282197) were purchased from Sigma-Aldrich, Bengaluru, India. Dulbecco's minimal essential media (DMEM) (12100-061), and fetal bovine serum (FBS, 10082147) were procured from Gibco, Thermo Fisher Scientific India Pvt. Ltd., Mumbai, India. LOX-5 (60402) was purchased from Cayman Chemical Company, USA. Sodium/potassium phosphate (RM 257, RM 249) and trichloroacetic acid (RM 6274) were procured from Himedia Ltd, Mumbai, India.