Cell culture

VM Valeria Manuelli
FC Fidelma Cahill
HW Harriet Wylie
CG Cheryl Gillett
IC Isabel Correa
SH Susanne Heck
AR Alex Rimmer
AH Anna Haire
MH Mieke Van Hemelrijck
SR Sarah Rudman
CW Claire M. Wells
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Human PCa cells 1532-CP2TX, 1535-CP1TX, and 1542-CP3TX [16] (referred to as CT-1532, -1535, -1542 cell lines) were cultured in Keratinocyte serum-free medium (KSFM) with 10% heat-inactivated fetal bovine serum, 1 mM penicillin–streptomycin, 25 μg/ml bovine pituitary extract (BPE), 5 ng/ml human EGF. Human pancreatic tumour cells AsPC-1 and PCa cells PC3 were cultured in RPMI-1640 media supplemented with 10% v/v HI-FBS and 1 mM penicillin–streptomycin.

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