Human PCa cells 1532-CP2TX, 1535-CP1TX, and 1542-CP3TX [16] (referred to as CT-1532, -1535, -1542 cell lines) were cultured in Keratinocyte serum-free medium (KSFM) with 10% heat-inactivated fetal bovine serum, 1 mM penicillin–streptomycin, 25 μg/ml bovine pituitary extract (BPE), 5 ng/ml human EGF. Human pancreatic tumour cells AsPC-1 and PCa cells PC3 were cultured in RPMI-1640 media supplemented with 10% v/v HI-FBS and 1 mM penicillin–streptomycin.
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