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Anthropometric and laboratory measurements

MZ Maryam Zarkesh
KT Kimia Tabaei
MA Mahdi Akbarzadeh
AD Afsoon Daneshafrooz
AZ Azita Zadeh-Vakili
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Weight, height, and waist, hip, wrist, and neck circumferences were measured according to the standard protocols, as described previously [15, 16]. Body mass index (BMI) was calculated as weight (kg) divided by square of height (m2). Systolic blood pressure (SBP) and diastolic blood pressure (DBP) were recorded using a standard sphygmomanometer after at least ten minutes of rest [17]. Blood samples were collected from all participants after 10-12 hours of overnight fasting. Fasting plasma glucose (FPG), triglyceride (TG), and total cholesterol (TC) levels were also measured using commercial kits (Pars Azmoon Inc., Tehran, Iran). Insulin was measured using the enzyme-linked immunosorbent assay (ELISA) with a Mercodia ELISA kit (Uppsala, Sweden). Inter-and intra-assay coefficients of variation (CVs) for FPG, TG, TC, and insulin were <5%. Finally, the subjects were divided into four groups, based on BMI: 18 normal weight (BMI <25 kg/m2), 19 overweight (BMI = 25–30 kg/m2), 18 obese (BMI = 30–40 kg/m2), and 19 morbidly obese (BMI≥40 kg/m2) individuals.

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