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Human umbilical vein endothelial cells (HUVEC) were purchased from Promocell, grown, and expanded in endothelial growth media-2 (EGM-2, Promocell, Germany). Primary human saphenous vein pericytes (SVP) were kindly donated from Prof Madeddu (University of Bristol) and cultured in EGM-2. SVP were grown and expanded in flasks or 35 mm dishes pre-coated with 0.1% fibronectin (Merck, United States) and 0.05% gelatin (Merck, United States) solution. Cells were maintained at 37°C with 5% CO2, passaged at 80% confluency and used up to passage 8 for experiments.
Copyright and License information: ©2022 Mastrullo, van der Veen, Gupta, Matos, Johnston, McVey, Madeddu, Velliou and Campagnolo.
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
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