Six-week-old juvenile male SHR and age- and sex-matched control Wistar Kyoto (WKY) rats weighing from 120 to 150 g were obtained from Shanghai Slac Laboratory Animal CO. LTD (Shanghai, China). SHR has been a well-established animal model for ADHD (at this age, SHRs do not develop hypertension) [6, 11] when WKYs have been used as the closest genetic control for the SHR. All the experiments were performed according to the Guidelines for the Care and Use of Laboratory Animals and approved by the Animal Ethics Committee of Fudan University Shanghai Medical College with the permit number of 20120302-003.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.