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Alexa 647-labeled siRNAs were complexed with Interferrin (PolyPlus) for 10 min in OptiMEM (siRNA final concentration = 10nM, Interferrin final volume/well/50 µL in 384-well plate format = 0.1µL), added to HeLa cells in 10% FBS and incubated for 30 min before washing and fixation. Fluorophore labeled siRNAs were ordered from Sigma Aldrich and sequences used are as follows. Sense strand: Alexa Fluor 647—5′-ACAUGAAGCAGCACGACUUdTdT-3′; antisense strand: 5′-AAGUCGUGCUGCUUCAUGUdTdT-3′.
Copyright and License information: ©2022
This article, published in RNA, is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.
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