Improve Research Reproducibility A Bio-protocol resource
- Home
- Protocols
-
Concise Method
Growth conditions for synchronous meiosis
Request a Protocol
Ask a
question
Favorite
Yeast strains were patched onto YP-Glycerol plates and transferred to YP-4%Dextrose plates. After this, cells were grown overnight in liquid YPD culture (room temperature) followed by inoculation in pre-sporulation media (BYTA; 50 mM Sodium Phthalate-buffered, 1% yeast extract, 2% tryptone, and 1% acetate) at OD600 = 0.3. Cells were grown for 18 hr in BYTA at 30°C, washed twice in water and resuspended in sporulation media (0.3% potassium acetate) at OD600 = 1.9 to induce meiosis at 30°C.
Copyright and License information: ©2021, Rousová et al
This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.
Post a Question0 Q&A
