Cell Proliferation

Cell proliferation was evaluated using MTT assay and colony formation assay. In the MTT assay, normal cancer cell lines and siRNA-transfected cell lines were cultured in 96-well plates at the same cell density. The MTT assay was performed every 8 hours, and the OD value at 458 nm was measured using a microplate spectrophotometer. The growth curve was drawn according to the OD value. In the colony formation assay, normal cancer cell lines and siRNA-transfected cell lines were cultured at the same cell density, immobilized with methanol and stained with crystal violet after 72 hours.