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Immunohistochemistry Assay

SL Shujun Lin
XJ Xuefeng Jiang
GZ Guijuan Zhang
XX Xinqin Xiao
XM Xinyi Ma
JW Jieyan Wu
DQ Dan Qiu
XL Xinyuan Li
XY Xianxin Yan
MM Min Ma
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The sections were dewaxed and hydrated, and endogenous peroxidase was blocked with hydrogen peroxide. Then antigen was retrieved by citrate buffer. After that, the slides were incubated with primary and secondary antibodies, used DAB staining, counterstained with hematoxylin buffer and sealing. Finally, the expression of CD34 in rat breast tissue was observed under Olympus BX53 microscope (Janpan) and analyzed with Image J software.

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