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qRT-PCR was performed with the SYBR Green I RNA-to-CT2-step kit (Vazyme) according to the manufacturer’s instructions and a CFX96 Touch real-time PCR detection system (Bio-Rad). The gyrB mRNA or 16s rRNA was served as Salmonella reference transcripts. Fold changes in expression were determined using the 2–ΔΔCt method (Ye et al., 2011). Primer sequences are given in Supplementary Table 2, and their specificities have been confirmed using Primer-BLAST (NCBI).
Copyright and License information: ©2022 Xue, Huang, Zhang, Hu, Pan, Peng, Lv, Meng and Li.
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