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Scratch Assay

RK Rajesh Kumari
RD Roshan Dutta
PR Prabhat Ranjan
ZS Zainab Gbongbo Suleiman
SG Sumanta Kumar Goswami
JL Jing Li
HP Harish Chandra Pal
SV Suresh Kumar Verma
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HMVE/HUVEC cells were seeded and were transfected with scramble or siALKBH5 siRNA for 24 hrs. There after, a straight scratch was lined using a 200 μl tip in the fully confluent monolayer. Pictures were taken using a phase-contrast microscope (Nikon Eclipse Inverted Phase Contrast Microscope, Spectra Services) and imaged at 20X magnification. The bar scale on image represents pixel size of 50 μM length. This was considered as zero time-point. Further, cell were incubated under normal or ischemic conditions for 24 h and pictures were retaken. The cellular migration rate was assessed as the area covered with time and data were analyzed using NIH ImageJ software and plotted (GraphPad PRISM software).

Copyright and License information:  ©2022 Kumari, Dutta, Ranjan, Suleiman, Goswami, Li, Pal and Verma.
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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