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Experiments With Drugs and Antibodies

GG Germán G. Gornalusse
MZ Mengying Zhang
RW Ruofan Wang
ER Emery Rwigamba
AK Anna C. Kirby
MF Michael Fialkow
EN Elizabeth Nance
FH Florian Hladik
LV Lucia Vojtech
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High Molecular Weight (HMW) Poly(I:C) was purchased from InvivoGen (catalog: tlrl-pic). A stock solution at 1 mg/ml was made using endotoxin free water. Ruxolitinib was obtained from Invivogen (catalog: tlrl-rux); a 20 mM stock solution was made with DMSO. Recombinant human IFN- λ1 (catalog: 300-02L, stock at 8 μg/ml), λ2 (catalog: 300-02K; stock at 8 μg/ml) and β (catalog: 3009-02BC; stock at 5 μg/ml) were from Peprotech. Cilengitide was from Adooq Bioscience; a 5 mM stock was made in DMSO. Mannan was from Sigma-Aldrich (catalog: M7504); a 100 mg/ml stock was made with PBS. Neutralizing antibodies: anti-AXL (R&D, catalog: AF154 RRID: AB_354852), anti-Tyro3 (R&D, catalog MAB859 RRID: AB_2210975), anti MerTK (R&D, catalog AF891 RRID: AB_355691). All drugs and antibodies were used at the concentrations and durations stated in the figure legends.

Copyright and License information:  ©2022 Gornalusse, Zhang, Wang, Rwigamba, Kirby, Fialkow, Nance, Hladik and Vojtech.
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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