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Paralysis assay

CD Chatrawee Duangjan
SC Sean P. Curran
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The CL4176 and CL2006 transgenic strain express human Aβ1–42 in muscle cells, as described previously [57, 58]. The transgenic worms were synchronized and treated with oolong tea extracts or OFs at the L4 stage on NGM agar plate. After treatment, CL4176 transgenic worms were maintained at 15 °C for 48 h and moved to 25 °C to induce Aβ expression. The number of paralyzed worms was counted at 20, 22, 24, 26, 28, and 30 h after moving to 25 °C. The CL2006 worms were maintained at 20 °C. Worms were classified as paralyzed when they did not move or only moved their head (cleared bacteria giving a halo appearance around the worms’ heads). Paralyzed worms were recorded and excluded from the plates every second day.

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