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Lentivirus-transfected (shCtrl, shCENPO) HCT116 and RKO cells were inoculated in 6-well plates (2 mL/well) for 5 days. After centrifugation, the cells were successively washed and precipitated by PBS and 1 × binding buffer, stained with 5 μL annexin V-APC in the dark for 15 min. The apoptosis rate was detected by flow cytometry and the results were analyzed by T test.
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