TKR-1 protein sequences ({"type":"entrez-protein","attrs":{"text":"NP_499064.2","term_id":"392896052","term_text":"NP_499064.2"}}NP_499064.2) were analyzed by database similarity search45 and the multiple protein sequences were simultaneously aligned using the COBALT, a constraint based alignment tool46. The transmembrane helix domains were predicted using the conserved domain database (CDD)47. For the phylogenetic tree analysis, Worm, human, rat, and mouse neurokinin receptor sequences used for alignment: worm TKR-1 ({"type":"entrez-protein","attrs":{"text":"NP_499064.2","term_id":"392896052","term_text":"NP_499064.2"}}NP_499064.2), h_TACR1/NK1R ({"type":"entrez-protein","attrs":{"text":"NP_001049.1","term_id":"4507343","term_text":"NP_001049.1"}}NP_001049.1), h_TACR2/NK2R ({"type":"entrez-protein","attrs":{"text":"NP_001048.2","term_id":"157426873","term_text":"NP_001048.2"}}NP_001048.2), h_TACR3/NK3R ({"type":"entrez-protein","attrs":{"text":"NP_001050.1","term_id":"7669548","term_text":"NP_001050.1"}}NP_001050.1), r_TACR1/NK1R ({"type":"entrez-protein","attrs":{"text":"NP_036799.1","term_id":"6981628","term_text":"NP_036799.1"}}NP_036799.1), r_TACR2/NK2R ({"type":"entrez-protein","attrs":{"text":"NP_542946.1","term_id":"18158435","term_text":"NP_542946.1"}}NP_542946.1), r_TACR3/NK3R ({"type":"entrez-protein","attrs":{"text":"NP_058749.1","term_id":"8394408","term_text":"NP_058749.1"}}NP_058749.1), m_TACR1/NK1R ({"type":"entrez-protein","attrs":{"text":"XP_006505926.1","term_id":"568941324","term_text":"XP_006505926.1"}}XP_006505926.1), m_TACR2/NK2R ({"type":"entrez-protein","attrs":{"text":"NP_067357.1","term_id":"10946720","term_text":"NP_067357.1"}}NP_067357.1), and m_TACR3/NK3R ({"type":"entrez-protein","attrs":{"text":"NP_033340.3","term_id":"258613887","term_text":"NP_033340.3"}}NP_033340.3). The phylogenetic tree was constructed by COBALT using minimum evolution method.
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