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RNA Extraction and Quantitative Reverse-Transcriptase Polymerase Chain Reaction
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Total RNA was extracted from rice panicles using RNAprep pure Plant Kit (TIANGEN, Beijing, China). Quantitative reverse-transcriptase polymerase chain reaction (qRT-PCR) was performed using SYBR Premix Ex Taq II (TAKARA, Dalian, China). Data analysis used the 2–ΔΔCt method, while the UBQ10 was used as the internal reference to normalize the gene expression (Livak and Schmittgen, 2001). The qRT-PCR primers used in this study are listed in Supplementary Table 1.
Copyright and License information: ©2022 Xue, Chen, Wen, Wang, Yang, Gong, Kang, Sun, Yu, Cao, Zhang, Zhan and Cheng.
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