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AAPT, NTPT, NTDT, AAPT-P, NTPT-P and NTDT-P samples (n = 4) were incubated in 1 ml of an S. mutans cell suspension at 37 °C for 1 and 24 h. All samples were rinsed with ddH2O three times, stained with 0.1% crystal violet solution for 15 min after fixation, washed with ddH2O and dried. The samples were rinsed until the excess crystal violet was removed. All samples were extracted in 1 ml of 95% ethanol and quantified by measuring the absorbance at 595 nm (A595).
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