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MIBI-TOF imaging

EM Erin F. McCaffrey
MD Michele Donato
LK Leeat Keren
ZC Zhenghao Chen
AD Alea Delmastro
MF Megan B. Fitzpatrick
SG Sanjana Gupta
NG Noah F. Greenwald
AB Alex Baranski
WG William Graf
RK Rashmi Kumar
MB Marc Bosse
CF Christine Camacho Fullaway
PR Pratista K. Ramdial
EF Erna Forgó
VJ Vladimir Jojic
DV David Van Valen
SM Smriti Mehra
SK Shabaana A. Khader
SB Sean C. Bendall
MR Matt van de Rijn
DK Daniel Kalman
DK Deepak Kaushal
RH Robert L. Hunter
NB Niaz Banaei
AS Adrie J. C. Steyn
PK Purvesh Khatri
MA Michael Angelo
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Imaging was performed using a MIBI-TOF instrument with a Hyperion ion source. Xe+ primary ions were used to sequentially sputter pixels for a given FOV. The following imaging parameters were used: acquisition setting, 80 kHz; field size, 500 μm x 500 μm (TB, M. avium and controls) or 450 μm × 450 μm (sarcoidosis) at 1,024 ×1,024 pixels; dwell time, 4 ms; median gun current on tissue, 1.45 nA Xe+; ion dose, 3.38 nAmp h per mm2 (500-μm2 FOVs) and 3.75 nAmp h per mm2 (450 μm2 FOVs).

For samples added to the cohort at a later time, the following imaging parameters were used: acquisition setting, 80 kHz; field size, 500 μm × 500 μm; dwell time, 1–2 ms; median gun current on tissue, 4.36 nA Xe+; ion dose, 2.54 × 5.08 nAmp h per mm2.

Copyright and License information: The Author(s) ©2022, corrected publication ©2022
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