Pictures of immunocytochemically stained cells were taken with a Cell Insight high content imaging platform (Thermo Fisher Scientific, Ltd) and a fluorescence microscope Axio Imager microscope (Carl Zeiss Inc.) using the Axio vision Digital Image Processing Software Version 4.67.1 (Carl Zeiss Inc.). Bright field images were taken with the Olympus inverted microscope using the same imaging software. Pictures were imported into ImageJ (http://rsbweb.nih.gov/ij/) for further processing.
Copyright and License information: The Author(s) ©2021 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
Search protocols in the Bio-protocol database
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this
article to respond.