2.3. Transwell Chamber Assay

YL Yongtao Li
WB Wenshan Bian
YJ Yang Jiang
DL Danyang Liu
LS Lei Shen
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This essay aimed to investigate whether hMSCs migrate to human islet B cell culture medium containing CXCL-13 in high-glucose environment.

Grouping was as follows: human islet B cell group and human islet B cell + CXCL-13 group.

Immortalized hMSCs were cultured in Transwell chamber, and human islet B cells were cultured in a lower layer culture plate. According to groups, they were incubated in an incubator at constant temperature for 10 h without or with CXCL-13. The observation index was the number of migrated cells.

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