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siRNA experiments were conducted using Lipofectamine RNAiMAX according to the manufacturer’s protocol, with small modifications. Adipocytes on day 5 of differentiation were treated with 60 pmole siRNA and 3 μL of lipofectamine for 1 well of a 24-well plate. siRNAs were SMARTpool ON-TARGETplus from Dharmacon (Trib1, L-057134-00-0005; Rap2a, L-041528-01-0005; and Nontargeting pool, D-001810-10-05). Media was not changed after transfection, and experiments were conducted 48 hours after transfection.
Copyright and License information: ©2022 Valentine et al.
This work is licensed under the Creative Commons Attribution 4.0 International License. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
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