Competitive transplants

HSCs were resuspended in 10% fetal bovine serum/phosphate-buffered saline supplemented with 2 × 10⁵ competitor BM cells. Cells were transplanted into lethally irradiated (900 cGy) mice via tail vein injection. PB was analyzed every 4 weeks for donor chimerism by using flow cytometry. For secondary transplants, BM was harvested from primary transplanted mice 16 weeks posttransplant, and 3 × 10⁶ BM cells were transplanted along with 2 × 10⁵ competitor BM cells. PB was analyzed every 4 weeks for donor chimerism.