Total RNA was extracted from glioma cells using TRIzol reagent (Life Technologies, Carlsbad, CA, USA). Reverse transcription was performed using 1 μg of total RNA and the High Capacity cDNA Reverse Transcription Kit (Toyobo, FSQ-101, Shanghai, China) according to the manufacturer’s protocol. An Mx-3000P Quantitative PCR System (Applied Biosystems, Foster City, USA) was used for qRT-PCR. The expression of GAPDH was used as a control to calibrate the original mRNA concentrations in tissues and cells. Target gene expression was calculated using the 2 -ΔΔCT method. The primer sequences are detailed in the Additional file Table S9.
Copyright and License information: The Author(s) ©2022 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this
article to respond.
Request a Protocol
Ask a
question
Post a Question
