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The active fraction from K. alvarezii were obtained from a previous study [14] using bioassay guided fractionation. Previous studies have succeeded in separating four fractions through the bioassay guided fractionation method (fraction I-IV), which we then selected the most active fraction as the active fraction (fraction II). This active fraction was used for the in vivo testing. The active fraction was determined based on its antiglycation ability using BSA-Glucose methods. The active fraction with highest antiglycation ability was used for the in vivo study.

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