Cell lines and Reagents

CV-1 (male) cells were obtained from ATCC, cultured at 37°C under 5% CO₂ in complete Dulbecco’s modified Eagle’s medium (DMEM), containing 10% fetal bovine serum (FBS), 10 U/ml penicillin, and 10 μg/ml streptomycin (GIBCO, Waltham, MA). DMEM, Opti-MEM, and 0.25% trypsin-EDTA were purchased from Thermo Fisher Scientific (Waltham, MA). FBS was purchased from R&D systems (Minneapolis, MN). Lipofectamine RNAiMax and ProLong Diamond Antifade Mountant with DAPI was purchased from Thermo Fisher Scientific (Waltham, MA). Phosphate-buffered saline (PBS) (1X) was purchased from GIBCO (Waltham, MA). Triton X-100, digitonin, and phenylmethylsulfonyl fluoride (PMSF) were purchased from Millipore Sigma (St. Louis, MO). Fugene HD was purchased from Promega (Madison, WI). Lunapark (Novus Biologicals, Centennial, CO), SV40 large T antigen (Santa Cruz Biotechnology, Santa Cruz, CA), SV40 VP2/3 and VP1 (Abcam, Cambridge, MA), Hsp90 (Santa Cruz Biotechnology, Santa Cruz, CA), BiP (Abcam, Cambridge, MA), and BAP31 (Thermo Fisher, Waltham, MA) antibodies were purchased from the indicated companies. The mCherry antibody is a generous gift from the lab of Dawen Cai (University of Michigan, Ann Arbor, MI). Details of the cell lines and reagents are listed in the Key resources table.