The serial concentrations of NGAL standards antigen (10, 50, 100, 200, 400, 800, 1500, 2000 and 3000 ng/mL) were prepared by using FBS to strengthen specific reaction of bioconjugate, each concentration done three replicates. After the clinical samples were added onto the sample pads, the results of fluorescence intensity on the T line (HT) and the C line (HC) were recorded by the reader. Quantitative detection was completed by the HT/HC ratio to effectively eliminate strips (T and C) difference and matrsample standard matrix effects[15]. The standard curve was plotted against each concentration of NGAL and HT/HC ratio.
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