2.3. Collection of apoptotic bodies

The isolation process involves a three‐step centrifugation. The initial centrifugation of the supernatant was at 300 × g for 10 min to remove cells. The supernatant was then subjected to a 3000 × g second centrifugation for 10 min to remove dead cells and debris. Finally, the remaining supernatant was subjected to a 10,000 × g ultracentrifugation for 60 min to obtain a pellet containing apoptotic bodies using an XPN‐100 ultracentrifuge (Beckman Coulter, USA). The pellet was resuspended in PBS for further study.