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Primer designing

SK Stuti Krishna
KM Kaushal Modha
VP Vipulkumar Parekh
RP Ritesh Patel
DC Digvijay Chauhan
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The GmPHYA3 sequence from Glycine max [3] was obtained in FASTA format from NCBI (National Centre for Biotechnology Information) GenBank database (https://www.ncbi.nlm.nih.gov/genbank/). GmPHYA3 is 9.2kb longer, exon 1 was divided into three frames and primers for each frame were designed using web-based Primer BLAST (Basic Local Alignment Search Tool) from NCBI (Table ​(Table1).1). These primers were utilized for amplification of PHYA3 in Lablab purpureus cv GNIB-21 which is determinate and photoperiod insensitive.

Primers used for partial amplification LprPHYA3 locus

F and R represent forward and reverse primers, respectively

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