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DL Daren Liu
YJ Yun Jin
JW Jinhong Wu
HZ Huanbing Zhu
DY Dan Ye
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The expression microarrays GSE41369 and GSE22780 of pancreatic cancer were obtained from Gene Expression Omnibus (GEO) database (https://www.ncbi.nlm.nih.gov/geo/). GSE41369, a miRNA expression microarray, includes 9 normal samples and 9 tumor samples. GSE22780, a gene expression microarray, includes 8 controls and 8 tumor samples. The R-language “limma” package was used for differential analysis. The screening criteria of the differentially expressed genes was |logFC| > 2, p < 0.05. Then, miRNAs and genes with significantly differential expression in pancreatic cancer were gained. The downstream target genes of miR-135b were predicted through miRDB, miRSearch, and TargetScan databases. Through STRING database, gene interaction analysis on candidate targets of miR-135b was conducted, and core value of each gene was counted. The core value refers to the ability of a gene to interact with other genes. The more the interacting genes, the higher the core value of this gene. Through consulting on hTFtarget database, the downstream regulatory genes of KLF4 were predicted.

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