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In brief, glands were extracted from 1 cm2 of human GC tissue using EDTA in cold chelation buffer, seeded in Matrigel (BD Biosciences) and overlaid with the medium as described to simulate the microenvironment of GC [48]. Exos-circRELL1 and circRELL1 plasmid were transfected into the organoids to access the exos-circRELL1 in GC progression. Human GC organoid growth was observed daily by microscope.
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