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According to the manufacturer's instructions, phagocytosis capacities of neutrophils were measured using a pHrodo E. coli BioParticles Phagocytosis kit (Thermo Fisher Scientific, USA). Neutrophils were diluted to 1 × 106 cells/mL buffer and incubated with the pHrodo E. coli particles at 37 °C and 5% CO2 for 0.5 h. Then, samples were centrifuged for 5 min. The phagocytosis of neutrophils was measured using flow cytometry.
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